Humicola lanuginosa lipase hydrolysis of mono-oleoyl-rac-glycerol at the lipid–water interface observed by atomic force microscopy
نویسندگان
چکیده
منابع مشابه
The role of Glu87 and Trp89 in the lid of Humicola lanuginosa lipase.
The importance of Glu87 and Trp89 in the lid of Humicola lanuginosa lipase for the hydrolytic activity at the water/lipid interface was investigated by site-directed mutagenesis. It was found that the effect on the hydrolytic activity upon the replacement of Trp89 with Phe, Leu, Gly or Glu was substrate dependent. The Trp89 mutants displayed an altered chain length specificity towards triglycer...
متن کاملMolecular machines directly observed by high-speed atomic force microscopy.
Molecular machines made of proteins are highly dynamic and carry out sophisticated biological functions. The direct and dynamic high-resolution visualization of molecular machines in action is considered to be the most straightforward approach to understanding how they function but this has long been infeasible until recently. High-speed atomic force microscopy has recently been realized, makin...
متن کاملImpact of the tryptophan residues of Humicola lanuginosa lipase on its thermal stability.
Thermal stability of wild type Humicola lanuginosa lipase (wt HLL) and its two mutants, W89L and the single Trp mutant W89m (W117F, W221H, and W260H), were compared. Differential scanning calorimetry revealed unfolding of HLL at T(d)=74.4 degrees C whereas for W89L and W89m this endotherm was decreased to 68.6 and 62 degrees C, respectively, demonstrating significant contribution of the above T...
متن کاملFluorescence spectroscopic characterization of Humicola lanuginosa lipase dissolved in its substrate.
The conformational dynamics of Humicola lanuginosa lipases (HLL) and its three mutants were investigated by steady state and time-resolved fluorescence spectroscopy in two different media, aqueous buffer and the substrate triacetin. The fluorescence of the four Trps of the wild-type HLL (wt) reports on the global changes of the whole lipase molecule. In order to monitor conformational changes s...
متن کاملNanoscale electrodes by conducting atomic force microscopy: oxygen reduction kinetics at the Ptmid R:CsHSO4 interface.
We quantitatively characterized oxygen reduction kinetics at the nanoscale Ptmid R:CsHSO(4) interface at approximately 150 degrees C in humidified air using conducting atomic force microscopy (AFM) in conjunction with AC impedance spectroscopy and cyclic voltammetry. From the impedance measurements, oxygen reduction at Ptmid R:CsHSO(4) was found to comprise two processes, one displaying an expo...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Biomembranes
سال: 2003
ISSN: 0005-2736
DOI: 10.1016/s0005-2736(03)00209-8